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cgrna hu6 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc cgrna hu6 plasmid
    Cgrna Hu6 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cgrna+hu6+plasmid/cgrna+hu6+plasmid/pmc06661866__oc9b00340_si_001-177-140-149
    Average 90 stars, based on 1 article reviews
    cgrna hu6 plasmid - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Modification:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    CRISPR:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Flow Cytometry:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Plasmid Preparation:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Transfection:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Binding Assay:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.

    Expressing:

    Article Title: Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology
    Article Snippet: S13 S1.3.2 Mammalian cell culture and induction assay for cgRNA studies Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.11–13 Briefly, four plasmids (Table S2) were co-transfected into HEK 293T cells and CRISPR/dCas9-mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).14 The reporter-gT1 plasmid was also a gift from G. Church (Addgene plasmid #47320)11 and was modified to replace the T1 gRNA target-binding region with the P1 gRNA target-binding region.12 Plasmid Function Fluorescent protein Parent plasmid ng/well Relative copy transfected number SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene 63798 16.4 0.2 reporter-gP1 P1 gRNA binding site upstream of a minimal CMV promoter expressing dTomato reporter dTomato Addgene 47320 233.6 6.8 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene 49016 32.0 1.0 trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene 49016 218.0 7.4 Table S2: Mammalian induction assay components.



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    Addgene inc cgrna hu6 plasmid
    Cgrna Hu6 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cgrna+hu6+plasmid/cgrna+hu6+plasmid/pmc06661866__oc9b00340_si_001-177-140-149
    Average 90 stars, based on 1 article reviews
    cgrna hu6 plasmid - by Bioz Stars, 2026-10
    90/100 stars
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